Because I measured my samples in duplicate, the duplicate measurements must be side-by-side one another, and they will automatically be averaged by Prism later on. Below the absorbance measurements of the standards, I have placed the absorbance measurements of my samples. The second two columns will contain the absorbance measurements for each of the standard curves, so here is the first standard curve, and this is the second one. The column first will contain the known concentration of your the standards, and their units and value depends completely on how you prepared your standard samples. So for typical ELISA data, you will want to have three columns. Prism does have tools of its own that can be used to manipulate your raw data, but my personal preference is to have the data in the following format before bringing it into Prism. In the last video, we demonstrated how to organize our data in Excel, such that it will be easy to copy into Prism for analysis. You can test it out with a free 30-day trial by clicking the link below or in the video description. If you have never used Prism before, I would definitely encourage you to try it.
I have been using Prism for a few years now, and it does a very good job of simplifying data analysis.
In this video, we will demonstrate one way of analyzing some typical ELISA data using GraphPad Prism.
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